Improvement in quality of cryopreserved human spermatozoa by swim-up before freezing
نویسندگان
چکیده
منابع مشابه
Improvement in motion characteristics and acrosome status in cryopreserved human spermatozoa by swim-up processing before freezing.
The purpose of this study was to examine if selecting a sperm population with improved motion characteristics before freezing reduces the deleterious effects of cryopreservation. Semen specimens from 15 normal donors were divided into two equal aliquots. The first aliquot received no treatment (control), and the second was processed by swim-up from a washed sperm preparation to select a sperm p...
متن کاملEffects of pentoxifylline treatment before freezing on motility, viability and acrosome status of poor quality human spermatozoa cryopreserved by the liquid nitrogen vapor method.
The objective of the present study was to investigate the effects of the direct addition of pentoxifylline (PF) to the ejaculates of men with poor sperm quality before freezing on post-thaw sperm motility, viability, acrosome integrity, and agonist-induced acrosome reaction. Semen specimens from 16 infertile men with impaired sperm count and motility (oligoasthenozoospermia) were divided into t...
متن کاملComparative study on density gradients and swim-up preparation techniques utilizing neat and cryopreserved spermatozoa.
AIM To 1) compare post-wash and post-thaw parameters of sperm processed with PureSperm density gradient technique and swim-up method; and 2) test the efficacy of two commonly available density gradient media PureSperm and ISolate. METHODS This prospective study used semen specimens from 22 patients. Specimens from nine patients were processed by both PureSperm density gradient and swim-up met...
متن کاملThe Effects of EGTA on the Quality of Fresh and Cryopreserved-Thawed Human Spermatozoa
Background: Sperm cryopreservation-thawing process has damaging effects on the structure and function of sperm, namely cryoinjury. Calcium overload has been reported as a postulated mechanism for sperm damage during the first steps after thawing. This study was designed to assess the intracellular calcium (Ca2+i) after cryopreservation and to clarify the role of a calcium chelator ethylene glyc...
متن کاملEffects of Rapid Cooling Prior to Freezing on the Quality of Canine Cryopreserved Spermatozoa
The aim of this study was to evaluate the effects of rapid cooling prior to freezing on frozen-thawed canine sperm quality. In experiment 1, centrifuged ejaculates from 6 dogs were pooled, split into 4 aliquots and cryopreserved by the Uppsala procedure using different cooling rates (control, cooling speed 18 C/90 min and average cooling rate 0.2 C/min; rapid, cooling speed 18 C/8 min and avera...
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ژورنال
عنوان ژورنال: International Journal of Andrology
سال: 1994
ISSN: 0105-6263,1365-2605
DOI: 10.1111/j.1365-2605.1994.tb01229.x